研究队伍

高巍

1. 个人简历:

高巍,男,博士,副教授,硕士生导师,目前,主要研究方向包括:1. 棉花纤维发育机制的研究;2. 棉花抗性相关基因的克隆及品种改良;3. 棉花CRISPR/Cas基因编辑技术的创新及应用。通过分子生物学研究和克隆棉花中纤维发育、抗生物和非生物逆境胁迫的优良基因,并解析其功能,对培育高产、多抗的棉花品种有理论意义和实践指导作用。先后参与主持国家自然科学基金项目,河南省科技创新杰出人才项目等国家和省部级重点项目多项,在Molecular & cellular proteomics、JXB、J Integr Plant Biol 等学术刊物发表论文十余篇,申请专利2项。

2. 教育经历:

2007-2014   华中农业大学  作物遗传育种  博士

2003-2007   华中农业大学  植物科学与技术 学士

3. 工作经历:

2017-至今   河南大学生命科学学院      副教授

2014-2017  河南大学生命科学学院      讲师

4. 承担项目:

(1)硝酸盐转运蛋白GbNRT2.1调节棉花抗旱的分子机理研究(国家自然科学基金青年项目,31601344,2017-2019)

(2)棉花抗盐相关基因的克隆和功能分析(国家重点研发计划,2016YFD0101006,2016-2020)参与

(3)GbMPK3介导的棉花抗黄萎病机制研究(国家自然科学基金青年项目,31701473,2018-2020)参与

(4)棉花高温胁迫下花药败育分子调控机理研究(国家自然科学基金青年项目,31401423,2015-2017)参与

(5)棉花纤维发育机制的蛋白质组学研究(河南省科技攻关计划,152102110163,2016-2017)参与

(6)棉纤维伸长基因的克隆及优良纤维棉花品种的选育(河南省教育厅科学技术研究重点项目,15A180028,2015-2016)

(7)GbMYB60的克隆和在棉花抗逆中的功能分析(棉花生物学国家重点实验室开放课题,CB2016A23,2016-2017)

5. 代表论文与著作:

(1)Lu Long**, Dan-Dan Guo**, Wei Gao, Wen-Wen Yang, Li-Pan Hou, Xiao-Nan Ma, Yu-Chen Miao, Jose Ramon Botella*, Chun-Peng Song*. Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression. Plant Methods 2018,

(2)Lu Long**, Jing-Ruo Zhao**, Fu-Chun Xu, Wen-Wen Yang, Peng Liao, Ya Gao, Wei Gao*, Chun-Peng Song. Silencing of GbANS reduces cotton resistance to Vertcillium dahliae through decreased ROS scavenging during the pathogen invasion process. Plant Cell, Tissue and Organ Culture (PCTOC) 2018,

(3)Fu-Chun Xu**, Hui-li Liu**, Yun-yun Xu, Jing-ruo Zhao, Ya-wei Guo, Lu Long, Wei Gao*, Chun-peng Song. Heterogeneous expression of the cotton R2R3-MYB transcription factor GbMYB60 increases salt sensitivity in transgenic Arabidopsis. Plant Cell, Tissue and Organ Culture (PCTOC) 2018, 133:15-25.

(4)Wei Gao†, Fu-Chun Xu†, Dan-Dan Guo, Jin-Ruo Zhao, Ji Liu, Ya-Wei Guo, Prashant K. Singh, Xiao-Nan Ma, Lu Long, Jose R. Botella, Chun-Peng Song*. Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress. BMC plant biology 2018, 18(1):15.

(5)Wei Gao**, Lu Long**, Xin-Quan Tian, Fu-Chun Xu, Ji Liu, Prashant K. Singh, Jose R. Botella, Chun-Peng Song*. Genome Editing in Cotton with the CRISPR/Cas9 System. Front Plant Sci 2017, 8:1364.

(6)Wei Gao, Lu Long, Xin-Quan Tian, Jing-Jing Jin, Hui-Li Liu, Hui Zhang, Fu-Chun Xu, Chun-Peng Song*. Genome-wide identification and expression analysis of stress associated proteins (SAPs) containing A20/AN1 zinc-finger in cotton. Mol Genet Genomics 2016, 291 (6):2199-2213.

(7)Wei Gao, Lu Long, Li Xu, Lindsey Keith, Xian-Long Zhang, Long-Fu Zhu*. Suppression of the homeobox gene HDTF1 enhances resistance to Verticillium dahliae and Botrytis cinerea in cotton. J Integr Plant Biol. 2016, 58(5):503-513.

(8)Wei Gao, Lu Long, Long-Fu Zhu*, Li Xu, Wen-Hui Gao, Long-Qing Sun, Lin-Lin Liu, Xian-Long Zhang*. Proteomic and virus-induced gene silencing (VIGS) analyses reveal that gossypol, brassinosteroids, and jasmonic acid contribute to the resistance of cotton to Verticillium dahliae. Molecular & cellular proteomics: MCP. 2013, 12 (12):3690-3703.

(9)Lu Long, Wei Gao, Li Xu, Min Liu, Xiang-Ying Luo, Xin He, Xi-Yan Yang, Xian-Long Zhang, Long-Fu Zhu*. GbMPK3, a mitogen-activated protein kinase from cotton, enhances drought and oxidative stress tolerance in tobacco. Plant Cell, Tissue and Organ Culture (PCTOC). 2014, 116 (2):153-162.

(10)Li Xu, Li Jin, Lu Long, Lin-Lin Liu, Xin He, Wei Gao, Long-Fu Zhu, Xian-Long Zhang*. Overexpression of GbWRKY1 positively regulates the Pi starvation response by alteration of auxin sensitivity in Arabidopsis. Plant Cell Reports, 2012, 31(12): 2177-2188.

(11)Li Xu, Long-Fu Zhu, Li-Li Tu, Xiao-Ping Guo, Lu Long, Long-Qing Sun, Wei Gao, Xian-Long Zhang*. Differential gene expression in cotton defence response to Verticillium dahliae by SSH. Journal of Phytopathology, 2011, 159(9): 606-615.

(12)高巍#, 刘会利#, 田新权, 张慧, 宋洁, 杨勇, 龙璐, 宋纯鹏*. 海岛棉转录因子基因GbMYB60的克隆、表达及其抗逆功能研究. 作物学报, 2016, 42(9): 1342-1351.

(13)杨勇#, 田新权#, 刘会利, 宋洁, 张慧, 龙璐, 高巍*. 海岛棉NAC转录因子基因GbNAC6的克隆和表达模式分析. 棉花学报, 2017, 29(2):138-146.

(14)张慧,田新权,高巍,蔡应繁,龙璐*. 陆地棉PPO的全基因组鉴定及对黄萎病菌的响应分析. 棉花学报, 2017, 29(5):428-436.

6. 专利:

(1)朱龙付,高巍,龙璐,张献龙,袁道军,聂以春。棉花硬脂酰去饱和化酶GbSSI2基因及应用. 2015年10月11日. 国家发明专利. CN201310019959.5。

(2)朱龙付,张献龙,高巍,龙璐。棉花同源结构域转录因子基因GbHDTF1及应用. 2015年10月11日. 国家发明专利. CN201310345168.1。

7. 联系方式:

(1)邮箱:gaowei021@163.com

(2)通讯地址:河南省开封市金明区河南大学生命科学学院