Cotton pedigree genome reveals restriction of cultivar-driven strategy in cotton breeding

作者：棉花生物育种与综合利用全国重点实验室 日期：2024-01-02 访问量：

Shang Liu, Dongyun Zuo, Hailiang Cheng, Man He, Qiaolian Wang, Limin Lv, Youping Zhang, Javaria Ashraf, Ji Liu, Guoli Song

Genome Biology

Doi:10.1186/s13059-023-03124-3

Abstract:

Background

Many elite genes have been identified from the available cotton genomic data, providing various genetic resources for gene-driven breeding. However, backbone cultivar-driven breeding is the most widely applied strategy. Revealing the genetic basis of cultivar-driven strategy’s restriction is crucial for transition of cotton breeding strategy.

Result

CRI12 is a backbone cultivar in cultivar-driven breeding. Here we sequence the pedigree of CRI12 using Nanopore long-read sequencing. We construct a graphical pedigree genome using the high-quality CRI12 genome and 13,138 structural variations within 20 different pedigree members. We find that low hereditary stability of elite segments in backbone cultivars is a drawback of cultivar-driven strategy. We also identify 623 functional segments in CRI12 for multiple agronomic traits in presence and absence variation-based genome-wide association study on three cohorts. We demonstrate that 25 deleterious segments are responsible for the geographical divergence of cotton in pathogen resistance. We also characterize an elite pathogen-resistant gene (GhKHCP) utilized in modern cotton breeding. In addition, we identify 386 pedigree fingerprint segments by comparing the segments of the CRI12 pedigree with those of a large cotton population.

Conclusion

We characterize the genetic patterns of functional segments in the pedigree of CRI12 using graphical genome method, revealing restrictions of cultivar-driven strategies in cotton breeding. These findings provide theoretical support for transitioning from cultivar-driven to gene-driven strategy in cotton breeding.